PHORBOL ESTERS INDUCE NITRIC-OXIDE SYNTHASE AND INCREASE ARGININE INFLUX IN CULTURED PERITONEAL-MACROPHAGES

被引：105

作者：

HORTELANO, S ^{[1
]}

GENARO, AM ^{[1
]}

BOSCA, L ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID, FAC FARM, INST BIOQUIM, E-28040 MADRID, SPAIN

来源：

FEBS LETTERS | 1993年 / 320卷 / 02期

关键词：

NITRIC OXIDE; PHORBOL ESTER; PROTEIN KINASE-C; MACROPHAGE;

D O I：

10.1016/0014-5793(93)80078-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of peritoneal macrophages with beta-phorbol 12,13-dibutyrate promotes a time-dependent release of NO to the incubation medium. This effect was antagonized by LPS, a well known inducer of nitric oxide synthase (NOS) expression in macrophages, and was inhibited by N(G)-methyl-L-arginine and N(omega)-nitro-L-arginine. An increase in intracellular cGMP and NOS activity was observed in parallel with NO release. The induction of NOS was accompanied by a stimulation of arginine influx within the cell. These results suggest that activation of protein kinase C by phorbol esters is sufficient to promote NOS induction in macrophages.

引用

页码：135 / 139

页数：5

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