IDENTIFICATION OF MESOPHILIC LACTIC-ACID BACTERIA BY USING POLYMERASE CHAIN REACTION-AMPLIFIED VARIABLE REGIONS OF 16S RIBOSOMAL-RNA AND SPECIFIC DNA PROBES

被引：219

作者：

KLIJN, N

WEERKAMP, AH

DEVOS, WM

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 11期

关键词：

D O I：

10.1128/AEM.57.11.3390-3393.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Specific DNA probes based on variable regions V1 and V3 of 16S rRNA of lactic acid bacteria were designed. These probes were used in hybridization experiments with variable regions amplified by using the polymerase chain reaction. In this way, a rapid and sensitive method was developed for the identification and classification of Lactococcus and Leuconostoc species.

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页码：3390 / 3393

页数：4

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