LIGHT MICROSCOPIC DETECTION OF LEUKOCYTE CELL-SURFACE ANTIGENS IN CELL SMEARS WITH IMMUNOGOLD-SILVER STAINING

被引：0
作者：
DEWAELE, M
RENMANS, W
SEGERS, E
DEVALCK, V
JOCHMANS, K
机构：
来源：
INSTITUTE OF PHYSICS CONFERENCE SERIES | 1990年 / 98期
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中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
An indirect immunogold-silver staining (IGSS) method for the light microscopical detection of leukocyte cell surface antigens in cell smears was developed. Labeling conditions were determined which gave a good morphology, an intense immunostaining and a low background. The lymphocyte subsets enumerated with this method in buffy coat smears of normal peripheral blood were comparable with those found with the alkaline phosphatase- anti alkaline phosphatase (APAAP) method. This IGSS procedure could form a general method for the detection of cell surface antigens in all kinds of cell smears.
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页码：671 / 674
页数：4
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共 8 条
  • [1] DETECTION OF CELL-SURFACE ANTIGENS IN CRYOSTAT SECTIONS WITH IMMUNOGOLD-SILVER STAINING
    DEWAELE, M
    DEMEY, J
    REYNAERT, P
    DEHOU, MF
    GEPTS, W
    VANCAMP, B
    [J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1986, 85 (05) : 573 - 578
  • [2] AN IMMUNOGOLD SILVER STAINING METHOD FOR DETECTION OF CELL-SURFACE ANTIGENS IN LIGHT-MICROSCOPY
    DEWAELE, M
    DEMEY, J
    RENMANS, W
    LABEUR, C
    REYNAERT, P
    VANCAMP, B
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1986, 34 (07) : 935 - 939
  • [3] ERBER WN, 1984, LANCET, V1, P1042
  • [4] SURFACE-MEMBRANE STAINING OF IMMUNOGLOBULINS IN PARAFFIN SECTIONS OF NON-HODGKINS LYMPHOMAS USING IMMUNOGOLD-SILVER STAINING TECHNIQUE
    HOLGATE, CS
    JACKSON, P
    LAUDER, I
    COWEN, PN
    BIRD, CC
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1983, 36 (07) : 742 - 746
  • [5] IMMUNOENZYMATIC STAINING OF HEMATOLOGICAL SAMPLES WITH MONOCLONAL-ANTIBODIES
    MOIR, DJ
    GHOSH, AK
    ABDULAZIZ, Z
    KNIGHT, PM
    MASON, DY
    [J]. BRITISH JOURNAL OF HAEMATOLOGY, 1983, 55 (03) : 395 - 410
  • [6] PALLESEN G, 1987, LEUKEMIA, V1, P231
  • [7] AN IMMUNOGOLD SILVER STAINING METHOD FOR THE LIGHT MICROSCOPIC ANALYSIS OF BLOOD LYMPHOCYTE SUBSETS WITH MONOCLONAL-ANTIBODIES
    ROMASCO, F
    ROSENBERG, J
    WYBRAN, J
    [J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1985, 84 (03) : 307 - 316
  • [8] CHARACTERIZATION OF HUMAN-LEUKOCYTE DIFFERENTIATION ANTIGENS
    SHAW, S
    [J]. IMMUNOLOGY TODAY, 1987, 8 (01): : 1 - 3
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