EVALUATION OF THE YEAST DEL ASSAY WITH 10 COMPOUNDS SELECTED BY THE INTERNATIONAL PROGRAM ON CHEMICAL SAFETY FOR THE EVALUATION OF SHORT-TERM TESTS FOR CARCINOGENS

被引：56
作者：
CARLS, N [1 ]
SCHIESTL, RH [1 ]
机构：
[1] HARVARD UNIV,SCH PUBL HLTH,DEPT MOLEC & CELLULAR TOXICOL,BOSTON,MA 02115
来源：
MUTATION RESEARCH | 1994年 / 320卷 / 04期
关键词：
YEAST DEL ASSAY; DEL ASSAY; EVALUATION OF SHORT-TERM TESTS; SACCHAROMYCES CEREVISIAE; SHORT-TERM TESTS; EVALUATION;
D O I：
10.1016/0165-1218(94)90082-5
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The Saccharomyces cerevisiae DEL assay detects a wide variety of nonmutagenic carcinogens (Schiestl et al. (1989) Carcinogenesis, 10, 1445-1455). This study shows the effect on DEL recombination of 8 carcinogenic compounds (o-toluidine, hexamethylphosphoramide, safrole, acrylonitrile, benzene, diethylhexylphthalate, phenobarbital and diethylstilbestrol) and 2 noncarcinogenic compounds (caprolactam and benzoin). These chemicals have been selected by the Program on Chemical Safety for the evaluation of short-term tests for carcinogens, because sufficient carcinogenicity data for these compounds exist, and because they are difficult to detect with the Salmonella assay. 5 of 8 carcinogens reproducibly gave a strong positive response and the noncarcinogen benzoin was negative. Thus, 60% of the chemicals tested in this study have been correctly identified with the DEL assay compared to only 20% with the Salmonella assay.
引用
收藏
页码：293 / 303
页数：11
相关论文
共 51 条
[1]   GENOTOXIC EFFECTS OF ETHYLENE-OXIDE AND PROPYLENE-OXIDE - A COMPARATIVE-STUDY [J].
AGURELL, E ;
CEDERBERG, H ;
EHRENBERG, L ;
LINDAHLKIESSLING, K ;
RANNUG, U ;
TORNQVIST, M .
MUTATION RESEARCH, 1991, 250 (1-2) :229-237
[2]   DEFINITIVE RELATIONSHIPS AMONG CHEMICAL-STRUCTURE, CARCINOGENICITY AND MUTAGENICITY FOR 301 CHEMICALS TESTED BY THE UNITED-STATES NTP [J].
ASHBY, J ;
TENNANT, RW .
MUTATION RESEARCH, 1991, 257 (03) :229-306
[3]   OVERVIEW OF THE GENETIC TOXICITY OF CAPROLACTAM AND BENZOIN [J].
ASHBY, J ;
SHELBY, MD .
MUTATION RESEARCH, 1989, 224 (03) :321-324
[4]  
ASHBY J, 1985, EVALUATION SHORT TER, P313
[5]   GENOTOXICITY STUDIES WITH THE UNSTABLE ZESTE-WHITE (UZ) SYSTEM OF DROSOPHILA-MELANOGASTER - RESULTS WITH 10 CARCINOGENIC COMPOUNDS [J].
BATISTEALENTORN, M ;
XAMENA, N ;
CREUS, A ;
MARCOS, R .
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1991, 18 (02) :120-125
[6]   THE MOLECULAR-GENETICS OF CANCER [J].
BISHOP, JM .
SCIENCE, 1987, 235 (4786) :305-311
[7]   CONSIDERATION OF BOTH GENOTOXIC AND NONGENOTOXIC MECHANISMS IN PREDICTING CARCINOGENIC POTENTIAL [J].
BUTTERWORTH, BE .
MUTATION RESEARCH, 1990, 239 (02) :117-132
[8]   THE ORIGIN OF HUMAN CANCERS [J].
CAIRNS, J .
NATURE, 1981, 289 (5796) :353-357
[9]  
de Serres F. J., 1981, PROGR MUTATION RES, V1
[10]   WERNERS SYNDROME - A REVIEW OF ITS SYMPTOMATOLOGY NATURAL HISTORY PATHOLOGIC FEATURES GENETICS AND RELATIONSHIP TO NATURAL AGING PROCESS [J].
EPSTEIN, CJ ;
MARTIN, GM ;
SCHULTZ, AL ;
MOTULSKY, AG .
MEDICINE, 1966, 45 (03) :177-+
123456