IN-VIVO FUNCTIONAL-ANALYSIS OF THE RAS EXCHANGE FACTOR SON OF SEVENLESS

被引：80
作者：
KARLOVICH, CA
BONFINI, L
MCCOLLAM, L
ROGGE, RD
DAGA, A
CZECH, MP
BANERJEE, U
机构：
[1] UNIV CALIF LOS ANGELES, DEPT BIOL, LOS ANGELES, CA 90024 USA
[2] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, LOS ANGELES, CA 90024 USA
[3] UNIV MASSACHUSETTS, MED CTR, PROGRAM MOLEC MED, WORCESTER, MA 01605 USA
[4] UNIV MASSACHUSETTS, MED CTR, DEPT BIOCHEM & MOLEC BIOL, WORCESTER, MA 01605 USA
来源：
SCIENCE | 1995年 / 268卷 / 5210期
关键词：
D O I：
10.1126/science.7725106
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Son of sevenless (Sos) protein functions as a guanine nucleotide transfer factor for Ras and interacts with the receptor tyrosine kinase Sevenless through the protein Drk, a homolog of mammalian Grb2. In vivo structure-function analysis revealed that the amino terminus of Sos was essential for its function in flies. A molecule lacking the amino terminus was a potent dominant negative. In contrast, a Sos fragment lacking the Drk binding sites was functional and its activity was dependent on the presence of the Sevenless receptor. Furthermore, membrane localization of Sos was independent of Drk. A possible role for Drk as an activator of Sos is discussed and a Drk-independent interaction between Sos and Sevenless is proposed that is likely mediated by the pleckstrin homology domain within the amino terminus.
引用
收藏
页码：576 / 579
页数：4
相关论文
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