TRANSPORT OF 3,5,3'-TRIIODOTHYRONINE INTO THE PERFUSED-RAT-LIVER AND SUBSEQUENT METABOLISM ARE INHIBITED BY FASTING

被引:35
作者
DEJONG, M [1 ]
DOCTER, R [1 ]
VANDERHOEK, HJ [1 ]
VOS, RA [1 ]
KRENNING, EP [1 ]
HENNEMANN, G [1 ]
机构
[1] ERASMUS UNIV, FAC MED, DEPT NUCL MED, 3000 DR ROTTERDAM, NETHERLANDS
关键词
D O I
10.1210/en.131.1.463
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The effects of 48-h fasting on transport of T3 and subsequent metabolism in the isolated perfused rat liver were investigated. Tracer T3 disappearance curves from the recirculating medium consisted of a fast component (FC) and a slow component (SC). Using a two-compartment model, both transport [expressed as the fractional transport rate constant from medium to liver (k21)] and disposal of T3 were calculated. After fasting, k21, total metabolism, and metabolism corrected for differences in mass transfer were diminished, pointing to both decreased transport and metabolism, presumably caused by depletion of liver ATP. Concerning transport, it was shown that only transport into the intracellular liver compartment and not transport to the extracellular liver compartment was decreased after fasting. As for metabolism, T3 glucuronidation was diminished; T3 sulfation and subsequent deiodination were not affected. All mentioned decreased parameters normalized after the addition of a combination of insulin, cortisol, and/or glucose to the medium, possibly by (partially) restoration of cellular energy stores.
引用
收藏
页码:463 / 470
页数:8
相关论文
共 46 条
[1]   EFFECT OF DIETARY COMPOSITION ON FASTING-INDUCED CHANGES IN SERUM THYROID-HORMONES AND THYROTROPIN [J].
AZIZI, F .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1978, 27 (08) :935-942
[2]   OBSERVATIONS ON THE FACTORS THAT CONTROL THE GENERATION OF TRIIODOTHYRONINE FROM THYROXINE IN RAT-LIVER AND THE NATURE OF THE DEFECT INDUCED BY FASTING [J].
BALSAM, A ;
INGBAR, SH .
JOURNAL OF CLINICAL INVESTIGATION, 1979, 63 (06) :1145-1156
[3]   INFLUENCE OF FASTING, DIABETES, AND SEVERAL PHARMACOLOGICAL AGENTS ON PATHWAYS OF THYROXINE METABOLISM IN RAT-LIVER [J].
BALSAM, A ;
INGBAR, SH .
JOURNAL OF CLINICAL INVESTIGATION, 1978, 62 (02) :415-424
[4]   THE INFLUENCE OF FASTING AND THE THYROID STATE ON THE ACTIVITY OF THYROXINE 5'-MONODEIODINASE IN RAT-LIVER - A KINETIC-ANALYSIS OF MICROSOMAL FORMATION OF TRIIODOTHYRONINE FROM THYROXINE [J].
BALSAM, A ;
SEXTON, F ;
INGBAR, SH .
ENDOCRINOLOGY, 1981, 108 (02) :472-477
[5]   IMPAIRED PERIPHERAL CONVERSION OF THYROXINE TO TRIIODOTHYRONINE [J].
CAVALIERI, RR ;
RAPOPORT, B .
ANNUAL REVIEW OF MEDICINE, 1977, 28 :57-65
[6]   ALTERATIONS IN MONODEIODINATION OF IODOTHYRONINES IN THE FASTING RAT - EFFECTS OF REDUCED NON-PROTEIN SULFHYDRYL-GROUPS AND HYPOTHYROIDISM [J].
CHOPRA, IJ .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1980, 29 (02) :161-167
[7]   EFFECT OF FASTING AND ACUTE ETHANOL ADMINISTRATION ON THE ENERGY-STATE OF INVIVO LIVER AS MEASURED BY P-31-NMR SPECTROSCOPY [J].
CUNNINGHAM, CC ;
MALLOY, CR ;
RADDA, GK .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 885 (01) :12-22
[8]  
DILLS RL, 1986, DRUG METAB DISPOS, V14, P190
[9]  
DILLS RL, 1986, J PHARMACOL EXP THER, V238, P463
[10]   DEVELOPMENT AND USE OF A MATHEMATICAL 2-POOL MODEL OF DISTRIBUTION AND METABOLISM OF 3,3',5-TRIIODOTHYRONINE IN A RECIRCULATING RAT-LIVER PERFUSION SYSTEM - ALBUMIN DOES NOT PLAY A ROLE IN CELLULAR-TRANSPORT [J].
DOCTER, R ;
DEJONG, M ;
VANDERHOEK, HJ ;
KRENNING, EP ;
HENNEMANN, G .
ENDOCRINOLOGY, 1990, 126 (01) :451-459