THE DROSOPHILA PROS-28.1 GENE IS A MEMBER OF THE PROTEASOME GENE FAMILY

被引:64
作者
HAASS, C [1 ]
PESOLDHURT, B [1 ]
MULTHAUP, G [1 ]
BEYREUTHER, K [1 ]
KLOETZEL, PM [1 ]
机构
[1] UNIV HEIDELBERG,ZMBH MOLEK GENET,IM NEUENHEIMER FELD 282,W-6900 HEIDELBERG,GERMANY
关键词
cDNA expression cloning; nuclear targeting signal; Proteasome; recombinant DNA; sequence comparison; tyrosine kinase;
D O I
10.1016/0378-1119(90)90185-T
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In the present communication, we report the identification of a new gene family which encodes the protein subunits of the proteasome. The proteasome is a high-Mr complex possessing proteolytic activity. Screening a Drosophila λgt11 cDNA expression library with the proteasome-specific antibody N19-28 we isolated a clone encoding the 28-kDa No. 1 proteasome protein subunit. In accordance with the nomenclature of proteasome subunits in Drosophila, the corresponding gene is designated PROS-28.1, and it encodes an mRNA of 1.1 kb with an open reading frame of 249 amino acids (aa). Genomic Southern-blot hybridization shows PROS-28.1 to be a member of a family of related genes. Analysis of the predicted aa sequence reveals a potential nuclear targeting signal, a potential site for tyrosine kinase and a potential cAMP/cGMP-dependent phosphorylation site. The aa sequence comparison of the products of PROS-28.1 and PROS-35 with the C2 proteasome subunit of rat shows a strong sequence similarity between the different proteasome subunits. The data suggest that at least a subset of the proteasome-encoding genes belongs to a family of related genes (PROS gene family) which may have evolved from a common ancestral PROS gene. © 1990.
引用
收藏
页码:235 / 241
页数:7
相关论文
共 39 条
[1]   SEA-URCHIN PROSOME - CHARACTERIZATION AND CHANGES DURING DEVELOPMENT [J].
AKHAYAT, O ;
DESA, FG ;
INFANTE, AA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (06) :1595-1599
[2]   IDENTITY OF THE 19S PROSOME PARTICLE WITH THE LARGE MULTIFUNCTIONAL PROTEASE COMPLEX OF MAMMALIAN-CELLS (THE PROTEASOME) [J].
ARRIGO, AP ;
TANAKA, K ;
GOLDBERG, AL ;
WELCH, WJ .
NATURE, 1988, 331 (6152) :192-194
[3]   CHARACTERIZATION OF THE PROSOME FROM DROSOPHILA AND ITS SIMILARITY TO THE CYTOPLASMIC STRUCTURES FORMED BY THE LOW-MOLECULAR WEIGHT HEAT-SHOCK PROTEINS [J].
ARRIGO, AP ;
DARLIX, JL ;
KHANDJIAN, EW ;
SIMON, M ;
SPAHR, PF .
EMBO JOURNAL, 1985, 4 (02) :399-406
[4]  
BEYREUTHER K, 1983, MODERN METHODS PROTE, P303
[5]  
COOPER JA, 1984, J BIOL CHEM, V259, P7835
[6]   ACTIVATION OF THE MULTICATALYTIC PROTEINASE FROM RAT SKELETAL-MUSCLE BY FATTY-ACIDS OR SODIUM DODECYL-SULFATE [J].
DAHLMANN, B ;
RUTSCHMANN, M ;
KUEHN, L ;
REINAUER, H .
BIOCHEMICAL JOURNAL, 1985, 228 (01) :171-177
[7]   THE MULTICATALYTIC PROTEINASE (PROSOME) IS UBIQUITOUS FROM EUKARYOTES TO ARCHAEBACTERIA [J].
DAHLMANN, B ;
KOPP, F ;
KUEHN, L ;
NIEDEL, B ;
PFEIFER, G ;
HEGERL, R ;
BAUMEISTER, W .
FEBS LETTERS, 1989, 251 (1-2) :125-131
[8]  
DAHLMANN B, 1989, INTRACELLULAR PROTEO, P217
[9]   PROTEIN IMPORT INTO THE CELL-NUCLEUS [J].
DINGWALL, C ;
LASKEY, RA .
ANNUAL REVIEW OF CELL BIOLOGY, 1986, 2 :367-390
[10]   DROSOPHILA SMALL CYTOPLASMIC 19S RIBONUCLEOPROTEIN IS HOMOLOGOUS TO THE RAT MULTICATALYTIC PROTEINASE [J].
FALKENBURG, PE ;
HAASS, C ;
KLOETZEL, PM ;
NIEDEL, B ;
KOPP, F ;
KUEHN, L ;
DAHLMANN, B .
NATURE, 1988, 331 (6152) :190-192