GAMMA-RAY MUTAGENESIS MEASUREMENT IN MAMMALIAN-CELLS

被引：15
作者：
PUCK, TT
HARVEY, WF
机构：
[1] UNIV COLORADO,CTR CANC,DENVER,CO 80262
[2] UNIV COLORADO,HLTH SCI CTR,DEPT MED,DENVER,CO 80262
来源：
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1995年 / 329卷 / 02期
关键词：
GAMMA-RAY MUTAGENESIS; LYMPHOCYTE MONITORING; REPAIR EFFECTIVENESS;
D O I：
10.1016/0027-5107(95)00028-H
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Application of conventional in vitro mutagenesis testing has so far failed to result in marked reduction of the total incidence of cancer. At least part of the reason may lie in the frequent use of a cell target too small to yield adequate sensitivity, and in failure to take into account the effects of cell killing in the assessment of mutagenic action. A single theoretical analysis fits the results of experimental data on gamma-irradiation applied to single marker gene testing in bacteria and to cytogenetic analysis of irradiated mammalian cells, and permits determination of the mean mutagenic dose, D-M(o), without complication due to cell killing. Cytogenetic monitoring of human lymphocytes which can detect mutagenic effects of gamma-radiation down to doses of < 0.1 Gy (10 rad) will also furnish an estimate of repair effectiveness at these low levels and may well be a useful tool in a program for prevention of cancer and other genetic disease.
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页码：173 / 181
页数：9
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