FOLLISTATIN BINDS TO BOTH ACTIVIN AND INHIBIN THROUGH THE COMMON BETA-SUBUNIT

被引:273
作者
SHIMONAKA, M
INOUYE, S
SHIMASAKI, S
LING, N
机构
[1] Department of Molecular Endocrinology, The Whittier Institute for Diabetes and Endocrinology, La Jolla, CA, 92037
关键词
D O I
10.1210/endo-128-6-3313
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Inhibin, activin, and follistatin are three families of polypeptides originally isolated and characterized from ovarian follicular fluid based on their modulation of FSH release from pituitary cell culture. In addition to their effects on FSH synthesis and secretion, inhibin and activin have other biological functions. By contrast, the physiological significance of follistatin was obscure, until it was discovered that follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex. Moreover, because inhibin contains a beta-subunit derived from activin, it is important to determine whether inhibin will also bind follistatin. Using a double-ligand blotting technique, we have determined that activin-A has two binding sites for follistatin, whereas inhibin-A has only one binding site for follistatin. Therefore, these results suggest that follistatin binds to both activin and inhibin through the common beta-subunit.
引用
收藏
页码:3313 / 3315
页数:3
相关论文
共 20 条
[1]   ACTIVIN-A MODULATES GROWTH-HORMONE SECRETION FROM CULTURES OF RAT ANTERIOR-PITUITARY-CELLS [J].
BILEZIKJIAN, LM ;
CORRIGAN, AZ ;
VALE, W .
ENDOCRINOLOGY, 1990, 126 (05) :2369-2376
[2]  
BURGER HG, 1988, ENDOCRINOLOGY, V122, P1701
[3]   PURIFICATION AND CHARACTERIZATION OF ERYTHROID-DIFFERENTIATION FACTOR (EDF) ISOLATED FROM HUMAN-LEUKEMIA CELL-LINE THP-1 [J].
ETO, Y ;
TSUJI, T ;
TAKEZAWA, M ;
TAKANO, S ;
YOKOGAWA, Y ;
SHIBAI, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 142 (03) :1095-1103
[4]   PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY [J].
GREENWOOD, FC ;
HUNTER, WM .
BIOCHEMICAL JOURNAL, 1963, 89 (01) :114-&
[5]   HETERODIMERS AND HOMODIMERS OF INHIBIN SUBUNITS HAVE DIFFERENT PARACRINE ACTION IN THE MODULATION OF LUTEINIZING HORMONE-STIMULATED ANDROGEN BIOSYNTHESIS [J].
HSUEH, AJW ;
DAHL, KD ;
VAUGHAN, J ;
TUCKER, E ;
RIVIER, J ;
BARDIN, CW ;
VALE, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :5082-5086
[6]   ACTIVIN-BINDING PROTEIN IS PRESENT IN PITUITARY [J].
KOGAWA, K ;
NAKAMURA, T ;
SUGINO, K ;
TAKIO, K ;
TITANI, K ;
SUGINO, H .
ENDOCRINOLOGY, 1991, 128 (03) :1434-1440
[7]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[8]  
LING N, 1990, CLIN OBSTET GYNECOL, P690
[9]   GONADAL AND EXTRAGONADAL EXPRESSION OF INHIBIN-ALPHA, INHIBIN-BETA-A, AND INHIBIN-BETA-B SUBUNITS IN VARIOUS TISSUES PREDICTS DIVERSE FUNCTIONS [J].
MEUNIER, H ;
RIVIER, C ;
EVANS, RM ;
VALE, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (01) :247-251
[10]   RAT FOLLISTATIN - GONADAL AND EXTRAGONADAL EXPRESSION AND EVIDENCE FOR ALTERNATIVE SPLICING [J].
MICHEL, U ;
ALBISTON, A ;
FINDLAY, JK .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (01) :401-407