PROTEIN-KINASE-C ACTIVITY AND SUBCELLULAR-DISTRIBUTION IN RAT-BRAIN FOLLOWING REPEATED ELECTROCONVULSIVE SEIZURES

被引：8

作者：

VERNET, O

ROSTWOROWSKI, K

SHERWIN, AL

机构：

[1] Montreal Neurological Institute, McGill University, Montreal

来源：

EXPERIMENTAL NEUROLOGY | 1992年 / 115卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0014-4886(92)90065-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Protein kinase C (PKC) activity was measured in samples of neocortex, cerebellum, and hippocampus from adult rats receiving a series of 10 electroconvulsive seizures (ECS). Rats were sacrificed immediately and at various intervals from 15 min to 24 h after the last seizure. From 77 to 84% of total PKC activity was found in the cytosol versus the membrane fraction. PKC activity in cerebellum was significantly higher than in neocortex (15%, P < 0.05). Repeated ECS treatment did not affect total PKC activity nor its distribution between membrane and cytosolic fractions when compared with sham ECS controls. This finding is in keeping with reports that adrenergic-stimulated phosphoinositol turnover is not altered 24 h following repeated ECS. © 1992.

引用

页码：297 / 301

页数：5

共 46 条

[1] PHOSPHORYLATION OF THE NEURONAL PROTEIN KINASE-C SUBSTRATE-B-50 - INVITRO ASSAY CONDITIONS ALTER SENSITIVITY TO ACTH [J].