The beta(1)-subunit is essential for modulation by protein kinase C of an human and a non-human L-type Ca2+ channel

被引：38

作者：

Bouron, A ^{[1
]}

Soldatov, NM ^{[1
]}

Reuter, H ^{[1
]}

机构：

[1] UNIV BERN,DEPT PHARMACOL,CH-3010 BERN,SWITZERLAND

来源：

FEBS LETTERS | 1995年 / 377卷 / 02期

关键词：

Xenopus oocyte; calcium channel; protein kinase C;

D O I：

10.1016/0014-5793(95)01327-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated in Xenopus oocytes the effects of phorbol ester-induced protein kinase C (PKC) stimulation on dihydropyridine (DHP)-insensitive and -sensitive Ca2+ channels. DHP-insensitive Ba2+ currents (I-Ba) were recorded from endogenous channels in non-injected oocytes and in oocytes injected with cRNAs encoding the auxiliary rabbit alpha(2)/delta and beta(1) Ca2+ channel subunits. A human alpha(1C) cRNA, injected alone or in combination with cRNAs of the auxiliary subunits, was used for studying DHP-sensitive I-Ba. We found that DHP-insensitive I-Ba was increased by 4 beta-phorbol 12-myristate 13-acetate (PMA), while DHP-sensitive I-Ba was decreased. In both cases, the effects depended only on the co-expression of the beta(1) subunit.

引用

页码：159 / 162

页数：4

共 22 条

[1] VOLTAGE-DEPENDENT FACILITATION OF A NEURONAL ALPHA(1C) L-TYPE CALCIUM-CHANNEL [J].