EFFECT OF IMMOBILIZATION ON STABILITY OF RECOMBINANT PLASMIDS IN GENE-ENGINEERED MICROORGANISMS

被引：7
作者：
ARIGA, O
WATARI, T
TAKEUCHI, K
ANDO, M
TAKAGI, H
SANO, Y
IIJIMA, S
KOBAYASHI, T
机构：
[1] SHINSHU UNIV, DEPT FINE MAT ENGN, 3-15-1 TOKIDA, UEDA, NAGANO 386, JAPAN
[2] NAGOYA UNIV, DEPT CHEM ENGN, NAGOYA, AICHI 464, JAPAN
来源：
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 1988年 / 21卷 / 02期
关键词：
BIOCHEMICAL ENGINEERING - CELL CULTURE - Growth Kinetics - MICROORGANISMS;
D O I：
10.1252/jcej.21.117
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
Stability of a recombinant plasmid pMCT98 was investigated for free and immobilized Escherichia coli C600/pMCT98 cells. KCl, a hardening reagent for immobilizing matrix, inhibited cell growth and decreased the stability of the plasmid for free cells. However, the immobilization within k-carrageenan gel did not affect cell growth and increased significantly the stability of the plasmid, and the amount of the gene product did not decrease through successive passage of the immobilized cells. Coexistence of plasmid pRLK13 lowered the stability of plasmid pMCT98 for both free and immobilized cells. Some oscillations in the ratio of cells harboring the plasmids were observed in the immobilized E. coli C600/pMCT98/pRLK13 system.
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页码：117 / 122
页数：6
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