INSITU HYBRIDIZATION IN HIV RESEARCH

被引：23
作者：
FOX, CH
COTTLERFOX, M
机构：
[1] NIH,WARREN G MAGNUSSON CLIN CTR,DEPT TRANSFUS MED,BETHESDA,MD 20892
[2] YALE UNIV,SCH MED,DEPT SURG,NEUROPATHOL SECT,NEW HAVEN,CT 06510
来源：
MICROSCOPY RESEARCH AND TECHNIQUE | 1993年 / 25卷 / 01期
关键词：
LENTIVIRUS; HIV; AIDS;
D O I：
10.1002/jemt.1070250111
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
In situ hybridization (ISH) for HIV is an arduous, demanding means of detecting viral genetic material in cells and tissues. Good ISH requires broad technical skills and devotion to controls for every step of the process as well as a critical eye when interpreting results. ISH may be used to detect HIV in three ways: by hybridizing to viral RNA, by hybridizing to proviral mRNA being produced for virion packaging, and by hybridizing to proviral DNA in the cytoplasm or integrated in the nucleus of an infected cell. Here we discuss the technical considerations involved and the problems encountered in using ISH to study the pathobiology of HIV infection.
引用
收藏
页码：78 / 84
页数：7
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