DOPAMINERGIC REGULATION OF DOPAMINE RELEASE FROM PC12 CELLS VIA A PERTUSSIS TOXIN-SENSITIVE G-PROTEIN

被引：24

作者：

COURTNEY, ND ^{[1
]}

HOWLETT, AC ^{[1
]}

WESTFALL, TC ^{[1
]}

机构：

[1] ST LOUIS UNIV,SCH MED,DEPT PHARMACOL,1402 S GRAND BLVD,ST LOUIS,MO 63104

来源：

NEUROSCIENCE LETTERS | 1991年 / 122卷 / 02期

关键词：

DOPAMINE; D2; RECEPTOR; PC12; CELL; PERTUSSIS TOXIN; G-PROTEIN; QUINPIROLE;

D O I：

10.1016/0304-3940(91)90873-R

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Regulation of dopamine (DA) release from PC12 cells was investigated. Apomorphine and quinpirole, a selective D2 agonist, significantly reduced K+-evoked DA release, and this reduction was reversed by haloperidol. Furthermore, spiroperidol, a selective D2 antagonist, and haloperidol, a nonselective DA antagonist, enhanced the K+-evoked DA release. Pertussis toxin treatment of the cells abolished the quinpirole-induced reduction of K+-evoked DA release. Also, the haloperidol-induced enhancement of K+-evoked DA release was not seen in pertussis toxin treated cells. These results, therefore, suggest the presence of D2 receptors on PC12 cells which result in the modulation of K+-evoked DA release via a pertussis toxin-sensitive G protein.

引用

页码：261 / 264

页数：4

共 24 条

[1] EVIDENCE FOR DOPAMINE RECEPTOR STIMULATION BY APOMORPHINE [J].

ANDEN, NE ;

RUBENSON, A ;

FUXE, K ;

HOKFELT, T .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 1967, 19 (09) :627-&

[2] A DOPAMINERGIC RECEPTOR MODULATES CATECHOLAMINE RELEASE FROM THE CAT ADRENAL-GLAND [J].

ARTALEJO, AR ;

GARCIA, AG ;

MONTIEL, C ;

SANCHEZGARCIA, P .

JOURNAL OF PHYSIOLOGY-LONDON, 1985, 362 (MAY) :359-368

[3] REGULATION OF DOPAMINE RELEASE FROM PC12 PHEOCHROMOCYTOMA CELL-CULTURES DURING STIMULATION WITH ELEVATED POTASSIUM OR CARBACHOL [J].

BAIZER, L ;

WEINER, N .

JOURNAL OF NEUROCHEMISTRY, 1985, 44 (02) :495-501

[4]

BEAN AJ, 1988, MOL PHARMACOL, V34, P715

[5] D-AMPHETAMINE-INDUCED INHIBITION OF CENTRAL DOPAMINERGIC-NEURONS - MEDIATION BY A STRIATO-NIGRAL FEEDBACK PATHWAY [J].

BUNNEY, BS ;

AGHAJANIAN, GK .

SCIENCE, 1976, 192 (4237) :391-393

[6]

BUNNEY BS, 1973, J PHARMACOL EXP THER, V185, P560

[7] PERTUSSIS TOXIN SUBSTRATE, THE PUTATIVE NI COMPONENT OF ADENYLYL CYCLASES, IS AN ALPHA-BETA-HETERODIMER REGULATED BY GUANINE-NUCLEOTIDE AND MAGNESIUM [J].

CODINA, J ;

HILDEBRANDT, J ;

IYENGAR, R ;

BIRNBAUMER, L ;

SEKURA, RD ;

MANCLARK, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (14) :4276-4280

[8]

FARNEBO LO, 1971, ACTA PHYSIOL SCAND, P35

[9]

GILMAN AG, 1987, ANNU REV BIOCHEM, V56, P615, DOI 10.1146/annurev.bi.56.070187.003151

[10] PERIPHERAL DOPAMINE-RECEPTORS - A CLASSIFICATION BASED ON POTENCY SERIES AND SPECIFIC ANTAGONISM [J].

GOLDBERG, LI ;

KOHLI, JD .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1983, 4 (02) :64-66

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