LIQUID-CHROMATOGRAPHIC DETERMINATION OF AZIDOTHYMIDINE IN HUMAN PLASMA USING ONLINE DIALYSIS AND PRECONCENTRATION OF A SILVER(I) - A THIOL STATIONARY PHASE

被引:14
作者
IRTH, H
DEJONG, GJ
LINGEMAN, H
BRINKMAN, UAT
机构
[1] Department of Analytical Chemistry, Free University, 1081 HV Amsterdam
关键词
Azidothymidine; Plasma;
D O I
10.1016/S0003-2670(00)83310-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An automated method is described for the routine determination of 3′-azido-2′,3′-dideoxythymidine (AZT), the best known drug against acquired immunodeficiency syndrome (AIDS). The method is based on on-line dialysis to remove matrix macromolecules, followed by selective preconcentration and clean-up with a silver(I)-thiol stationary phase. After desorption of the solute with a small plug of perchloric acid, chromatography is applied using an octadecyl-modified silica column. Using UV absorbance detection at 269 nm, the minimum detectable concentration in plasma is 20 ng ml-1 (600-μl sample). The within-day reproducibility at the 20 ng ml-1 level is 4.4% and at least 128 samples can be analysed unattendedly without exchanging the dialysis membrane, the precolumn or the analytical column. © 1990.
引用
收藏
页码:165 / 172
页数:8
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