ROLE OF THE FAR-UPSTREAM SITES OF THE ALGD PROMOTER AND THE ALGR AND RPON GENES IN ENVIRONMENTAL MODULATION OF MUCOIDY IN PSEUDOMONAS-AERUGINOSA

被引：62
作者：
MOHR, CD
MARTIN, DW
KONYECSNI, WM
GOVAN, JRW
LORY, S
DERETIC, V
机构：
[1] UNIV TEXAS,HLTH SCI CTR,DEPT MICROBIOL,SAN ANTONIO,TX 78284
[2] UNIV WASHINGTON,SCH MED,DEPT MICROBIOL,SEATTLE,WA 98195
来源：
JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 11期
关键词：
D O I：
10.1128/jb.172.11.6576-6580.1990
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The role of several regulatory elements in environmental modulation of mucoidy in Pseudomonas aeruginosa was studied. Transcriptional activation of algD, necessary for the mucoid phenotype, was found to depend on FUS, the newly identified far-upstream sites of the algD promoter. The FUS were delimited to a region spanning nucleotides -432 to -332 relative to the algD mRNA start site. Insertional inactivation of algR in PAO568 abolished the algD promoter response to nitrogen availability and greatly diminished but did not completely eliminate reactivity to changes in salt concentration. Insertional inactivation of rpoN (ntrA) in PAO568 did not affect algR and algD transcription.
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页码：6576 / 6580
页数：5
相关论文
共 27 条
[1]   HIGH OSMOLARITY IS A SIGNAL FOR ENHANCED ALGD TRANSCRIPTION IN MUCOID AND NONMUCOID PSEUDOMONAS-AERUGINOSA STRAINS [J].
BERRY, A ;
DEVAULT, JD ;
CHAKRABARTY, AM .
JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2312-2317
[2]   UPSTREAM ACTIVATOR SEQUENCES ARE PRESENT IN THE PROMOTERS OF NITROGEN-FIXATION GENES [J].
BUCK, M ;
MILLER, S ;
DRUMMOND, M ;
DIXON, R .
NATURE, 1986, 320 (6060) :374-378
[3]  
CHENG PW, J CLIN INVEST, V85, P68
[4]   A RAPID SINGLE-STRANDED CLONING STRATEGY FOR PRODUCING A SEQUENTIAL SERIES OF OVERLAPPING CLONES FOR USE IN DNA SEQUENCING - APPLICATION TO SEQUENCING THE CORN MITOCHONDRIAL 18-S RDNA [J].
DALE, RMK ;
MCCLURE, BA ;
HOUCHINS, JP .
PLASMID, 1985, 13 (01) :31-40
[5]   GENE ALGD CODING FOR GDPMANNOSE DEHYDROGENASE IS TRANSCRIPTIONALLY ACTIVATED IN MUCOID PSEUDOMONAS-AERUGINOSA [J].
DERETIC, V ;
GILL, JF ;
CHAKRABARTY, AM .
JOURNAL OF BACTERIOLOGY, 1987, 169 (01) :351-358
[6]   THE ALGR GENE, WHICH REGULATES MUCOIDY IN PSEUDOMONAS-AERUGINOSA, BELONGS TO A CLASS OF ENVIRONMENTALLY RESPONSIVE GENES [J].
DERETIC, V ;
DIKSHIT, R ;
KONYECSNI, WM ;
CHAKRABARTY, AM ;
MISRA, TK .
JOURNAL OF BACTERIOLOGY, 1989, 171 (03) :1278-1283
[7]  
DERETIC V, 1989, Bio-Technology (New York), V7, P1249
[8]   PSEUDOMONAS-AERUGINOSA INFECTION IN CYSTIC-FIBROSIS - NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL REGULATION OF THE ALGD GENE [J].
DERETIC, V ;
GILL, JF ;
CHAKRABARTY, AM .
NUCLEIC ACIDS RESEARCH, 1987, 15 (11) :4567-4581
[9]   MUCOID PSEUDOMONAS-AERUGINOSA IN CYSTIC-FIBROSIS - MUTATIONS IN THE MUC LOCI AFFECT TRANSCRIPTION OF THE ALGR AND ALGD GENES IN RESPONSE TO ENVIRONMENTAL STIMULI [J].
DERETIC, V ;
GOVAN, JRW ;
KONYECSNI, WM ;
MARTIN, DW .
MOLECULAR MICROBIOLOGY, 1990, 4 (02) :189-196
[10]   CONTROL OF MUCOIDY IN PSEUDOMONAS-AERUGINOSA - TRANSCRIPTIONAL REGULATION OF ALGR AND IDENTIFICATION OF THE 2ND REGULATORY GENE, ALGQ [J].
DERETIC, V ;
KONYECSNI, WM .
JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :3680-3688
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