Development and validation of RP-HPLC method for the determination of Cefazolin

A RP-HPLC assay method has been developed and validated for estimation cefazolin. An isocratic RP-HPLC method developed on a SS Wakosil II-C-18 column (250 mm x 4.6 mm i.d., 5 mu m) with a mobile phase of phosphate buffer (pH 6.8) and methanol (5:2 v/v) and UV detection at 254 nm at flow rate 1 ml/min. The response of drug was linear form the conc. ranges 1-100 mu g/ml the no. of theoretical plates and tailing factor were 3745.04 and 1 respectively. Limit of detection and Limit of quantification were found to be 0.1 mu g/ml and 0.2 mu g/ml respectively. The percentage recovery ranges from 96-105%. Method precision and precision of the system was within the limits of acceptance criteria. The method was found to be robust in changed chromatographic conditions. The established method can be applied to the assay of marketed cefazolin injection formulation.