EVALUATION OF DNA PROBES FOR SPECIFIC DETECTION OF VIBRIO-CHOLERAE-O139 BENGAL

被引：17
作者：
NAIR, GB
BAG, PK
SHIMADA, T
RAMAMURTHY, T
TAKEDA, T
YAMAMOTO, S
KURAZONO, H
TAKEDA, Y
机构：
[1] INT MED CTR JAPAN,RES INST,SHINJUKU KU,TOKYO 162,JAPAN
[2] NATL INST CHOLERA & ENTER DIS,CALCUTTA 700010,W BENGAL,INDIA
[3] NATL INST HLTH,ENTER INFECT LAB,SHINJUKU KU,TOKYO 162,JAPAN
[4] NATL CHILDRENS MED RES CTR,DEPT INFECT DIS RES,SETAGAYA KU,TOKYO 154,JAPAN
[5] KYOTO UNIV,FAC MED,DEPT MICROBIOL,SAKYO KU,KYOTO 60601,JAPAN
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 08期
关键词：
D O I：
10.1128/JCM.33.8.2186-2187.1995
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two DNA probes, 2R1 and 2R3, prepared from a region in the chromosome specific for the lipopolysaccharide O side chains of Vibrio cholerae O139 (M. and Waldor and J. J. Mekalanos, Lancet 343:1366, 1994) were examined for their specificity and sensitivity. Both probes did not hybridize with any strain of V. cholerae belonging to serogroups other than O139 and to any of the other species examined belonging to the family Vibrionaceae. Among the 126 strains of V. cholerae O139 examined, probe 2R1 hybridized with 125 strains while probe 2R3 hybridized with ail 126 strains. Both probes were found to be highly specific and sensitive and can be used for the specific identification of V. cholerae O139.
引用
收藏
页码：2186 / 2187
页数：2
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