Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine

被引：28
作者：
Ahadi, Ali [1 ]
Partoazar, Alireza [2 ]
Abedi-Khorasgani, Mohammad-Hassan [4 ]
Shetab-Boushehri, Seyed Vahid [3 ]
机构：
[1] Univ Tehran Med Sci, Sch Med, Dept Physiol, Tehran, Iran
[2] Univ Tehran Med Sci, Sch Med, Dept Pharmacol, Tehran, Iran
[3] Univ Tehran Med Sci, Sch Med, Dept Med Nanotechnol, POB 14155-5983, Tehran, Iran
[4] Legal Med Org Iran, Tehran, Iran
来源：
JOURNAL OF BIOMEDICAL RESEARCH | 2011年 / 25卷 / 05期
关键词：
morphine detection; liquid-liquid extraction; thin-layer chromatography; solid-phase extraction; high-performance thin layer chromatography;
D O I：
10.1016/S1674-8301(11)60048-1
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Liquid-liquid extraction-thin layer chromatography (LLE-TLC) has been a common and routine combined method for detection of drugs in biological materials. Solid-phase extraction (SPE) is gradually replacing the traditional LLE method. High performance thin layer chromatography (HPTLC) has several advantages over TLC. The present work studied the higher efficiency of a new SPE-HPTLC method over that of a routine LLE-TLC method, in extraction and detection of urinary morphine. Fifty-eight urine samples, primarily identified as morphine-positive samples by a strip test, were re-screened by LLE-TLC and SPE-HPTLC. The results of LLE-TLC and SPE-HPTLC were then compared with each other. The results showed that the SPE-HPTLC detected 74% of total samples as morphine-positive samples whereas the LLE-TLC detected 48% of the same samples. We further discussed the effect of codeine abuse on TLC analysis of urinary morphine. Regarding the importance of morphine detection in urine, the present combined SPE-HPTLC method is suggested as a replacement method for detection of urinary morphine by many reference laboratories.
引用
收藏
页码：362 / 367
页数：6
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