A BIOSENSOR AS WARNING DEVICE FOR THE DETECTION OF CYANIDE, CHLOROPHENOLS, ATRAZINE AND CARBAMATE PESTICIDES

被引：114
作者：
BESOMBES, JL
COSNIER, S
LABBE, P
REVERDY, G
机构：
[1] UNIV GRENOBLE 1,ELECTROCHIM ORGAN & PHOTOCHIM REDOX LAB,CNRS,URA 1210,F-38041 GRENOBLE 9,FRANCE
[2] UNIV SAVOIE,ESIGEC,CHIM MOLEC & ENVIRONNEMENT LAB,F-73376 LE BOURGET DU LAC,FRANCE
来源：
ANALYTICA CHIMICA ACTA | 1995年 / 311卷 / 03期
关键词：
BIOSENSORS; ENZYMATIC METHODS; TYROSINASE ELECTRODE; CYANIDE; ENVIRONMENTAL ANALYSIS; PESTICIDES; CHLOROPHENOLS; WATER POLLUTANTS;
D O I：
10.1016/0003-2670(94)00686-G
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The determination of cyanide, chlorophenols, atrazine, dithiocarbamate and carbamate pesticides is described, utilizing an amperometric biosensor constructed by the electropolymerization of a pyrrole amphiphilic monomer-tyrosinase coating. Measurements were carried out with catechol, dopamine, L-DOPA or epinephrine as an enzyme substrate; the enzymatically generated quinoid products being electroreduced at -200 mV vs. SCE. The detection of these water pollutants was performed via their inhibiting action on the tyrosinase electrode. The characterization of the inhibition processes (competitive/non-competitive) and their reversibility were examined. The detection limits are 0.4, 2, 2, 4 and 0.02 mu M for 3,4-dichlorophenol, chloroisopropylphenylcarbamate, 3-chloroaniline, atrazine and cyanide, respectively.
引用
收藏
页码：255 / 263
页数：9
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