A THIOSTREPTON-INDUCIBLE EXPRESSION VECTOR FOR USE IN STREPTOMYCES-SPP

被引：21

作者：

KUHSTOSS, S

RAO, RN

机构：

[1] Lilly Research Laboratories, Lilly Corporate Center, Indianapolis

来源：

GENE | 1991年 / 103卷 / 01期

关键词：

RECOMBINANT DNA; HYGROMYCIN-B RESISTANCE; SHUTTLE VECTOR; TIPA; XYLE;

D O I：

10.1016/0378-1119(91)90398-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A shuttle expression vector containing the thiostrepton-inducible Streptomyces lividans promoter, p(tipA), and the origin of transfer from plasmid RP4 was constructed. Cassettes containing a promoterless xylE gene upstream from a hyg gene were used to demonstrate thiostrepton-inducible expression from p(tipA) in both S. lividans and Streptomyces ambofaciens. p(tipA) was estimated to be induced 60-fold or more in Streptomyces ambofaciens.

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页码：97 / 99

页数：3

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