PH-DEPENDENCE OF OXIDATION-REDUCTION POTENTIAL OF CYTOCHROME C-2

被引：24
作者：
PETTIGREW, GW
MEYER, TE
BARTSCH, RG
KAMEN, MD
机构：
[1] UNIV CALIF SAN DIEGO, DEPT CHEM, LA JOLLA, CA 92093 USA
[2] UNIV SO CALIF, BIOL DEV LAB, LOS ANGELES, CA 90007 USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1976年 / 430卷 / 02期
关键词：
D O I：
10.1016/0005-2728(76)90079-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The pH dependence of the spectra and of the oxidation-reduction potential of 3 cytochromes c2, from Rhodopseudomonas [R.] capsulata, R. sphaeroides and Rhodomicrobium vannielii, were studied. A single alkaline pK was observed for the spectral changes in all 3 ferricytochromes. In R. capsulata cytochrome c2 this spectroscopic pK corresponds to the pK observed in the dependence of oxidation-reduction potential on pH. For the other 2 cytochromes the oxidation-reduction potential showed a complex dependency on pH which can be fitted to theoretical curves involving 3 ionizations. The 3rd ionization corresponds to the ionization observed in the spectroscopic studies but the 1st 2 occur without changes in the visible spectra. The possible structural bases for these ionizations are discussed.
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页码：197 / 208
页数：12
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