CD8(+) LYMPHOCYTES IN BRONCHOALVEOLAR LAVAGE AND BLOOD - IN-VIVO INDICATORS OF LUNG PATHOLOGY CAUSED BY MAEDI-VISNA VIRUS

A study to determine the putative relationship between lymphocyte phenotypic alterations in bronchoalveolar lavage fluid and stage of lung pathology in maedi-visna infected sheep has been carried out. Twenty-one ewes (16 Texel and five Scottish blackface) naturally infected by maedi-visna virus and three Oxford controls were used. Animals were killed, lungs were removed, bronchoalveolar lavage was performed and pathological studies were completed. Blood samples were also obtained from 16 animals. Lymphocytes in both bronchoalveolar lavage and peripheral blood were labelled with monoclonal antibodies against the main T lymphocyte subsets (CD4, CD8, CD5 and gamma delta TCR) in order to perform flow cytometric studies. Three aspects of pathology were studied: lymphoid interstitial pneumonia, lymphoid follicular hyperplasia and smooth muscle hyperplasia. Percentages of CD4(+), CD5(+), gamma delta(+) T cells and the value for the CD4(+)/CD8(+) ratio in bronchoalveolar lavage of maedi-visna infected animals were significantly decreased (P < 0.05) when compared to controls, while percentages of CD8(+) lymphocytes were increased in bronchoalveolar lavage of infected sheep and they were very close to being significant (P = 0.07) when compared to controls. Lesions were evaluated and simple least-squares regression tests demonstrated that there were several significant correlations between various lymphocyte subsets and pathological parameters studied in this work. However, when a multiple regression test was applied to the data, it was observed that only the CD8(+) T cell subset both in bronchoalveolar lavage and in blood was significantly correlated with severity of lung pathology. It is concluded that CD8(+) lymphocytes are key cells in the development of the interstitial reaction and the lymphocytic alveolitis observed in maedi-visna infected ewes and that the CD8(+) alveolitis is a parallel feature to the intensity of lung lesions. It is further suggested that the percentage of CD8(+) lymphocytes in bronchoalveolar lavage and in blood may act as in vivo indicators of lung pathology in maedi-visna infected sheep.