Developing cell-free protein synthesis systems: a focus on mammalian cells

被引：9

作者：

Broedel, Andreas K. ^{[1
,2
]}

Kubick, Stefan ^{[1
]}

机构：

[1] Fraunhofer Inst Cell Therapy & Immunol IZI, Branch Bioanalyt Bioproc Potsdam Golm IZI BB, Am Muhlenberg 13, D-14476 Potsdam, Germany

[2] Imperial Coll London, Dept Life Sci, London SW7 2AZ, England

来源：

PHARMACEUTICAL BIOPROCESSING | 2014年 / 2卷 / 04期

关键词：

D O I：

10.4155/PBP.14.30

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sophisticated cell-free protein synthesis (CFPS) systems have been developed as an alternative to recombinant expression in cultured cells. In this review, we present advances in the field of mammalian-based CFPS by highlighting recently established systems derived from mouse fibroblasts, HeLa, hybridoma, CHO and K562 cells. We further highlight ongoing challenges in the field of mammalian-based CFPS, such as the optimization of already established platforms and the development of novel systems in order to further increase protein yields and reduce manufacturing costs while facilitating the synthesis of a huge number of biologically active target proteins. Advances in mammalian-based CFPS shall expand the number of future applications of CFPS in the area of pharmaceutical research and development.

引用

页码：339 / 348

页数：10

共 93 条

[91] Microscale to Manufacturing Scale-up of Cell-Free Cytokine Production-A New Approach for Shortening Protein Production Development Timelines [J].