HETEROGENEITY AMONG HEAT-LABILE ENTEROTOXINS PRODUCED BY PORCINE ENTEROTOXIGENIC ESCHERICHIA-COLI

被引：0

作者：

TSUJI, T

YAO, S

MIYAMA, A

机构：

[1] Department of Microbiology, Fujita Health University School of Medicine, Toyoake Aichi

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1992年 / 8卷 / 04期

关键词：

HEAT-LABILE ENTEROTOXINS (LT); A-SUBUNIT AND B-SUBUNIT; PI VALUE;

D O I：

10.1007/BF00146374

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The heat-labile enterotoxins (LT) produced by various porcine strains (LTp) of enterotoxigenic Escherichia coli were purified to homogeneity and their molecular properties were compared with each other. By double gel diffusion and rabbit skin permeability test, LTps from WT-1 (LTp-WT-1) and BO-149 (LTp-BO-149) strains were antigenically and biologically identical. By polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS), the mobilities of A and B subunits of LTp (BO-149) were identical to those of LTp (WT-1). However, on polyacrylamide gel electrophoresis without SDS, LTp (BO-149) and LTp (WT-1) differed in mobility, suggesting their molecular differences. Though the pI value of the B subunit of LTp (BO-149) was identical to that of LTp (WT-1), the pI value of the A subunit of LTp (BO-149) was higher than that of LTp (WT-1). These data suggest that there is molecular heterogeneity among LTps produced by the two porcine enterotoxigenic Escherichia coli strains.

引用

页码：543 / 547

页数：5

共 21 条

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