AN ACID-LABILE LINKER FOR SOLID-PHASE OLIGORIBONUCLEOTIDE SYNTHESIS USING FMOC GROUP FOR 5'-HYDROXYL PROTECTION

被引：7

作者：

PALOM, Y ^{[1
]}

ALAZZOUZI, E ^{[1
]}

GORDILLO, F ^{[1
]}

GRANDAS, A ^{[1
]}

PEDROSO, E ^{[1
]}

机构：

[1] UNIV BARCELONA,DEPT QUIM ORGAN,MARTI I FRANQUES 1-11,E-08028 BARCELONA,SPAIN

来源：

TETRAHEDRON LETTERS | 1993年 / 34卷 / 13期

关键词：

OLIGORIBONUCLEOTIDE SYNTHESIS; SOLID-PHASE; ACETAL LINKER; FMOC; MTHP;

D O I：

10.1016/S0040-4039(00)60380-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

An alkoxybenzylidene acetal linker formed with the 2'- and 3'-OH of the 3'-terminal ribonucleoside and attached to the solid support through an amide bond fulfills the necessary requirements of base stability and acid lability to be used in solid phase oligoribonucleotide synthesis in combination with Fmoc and Mthp groups for 5'-OH and 2'-OH protection respectively . Methyl protected phosphates do not give rise to N-methylation of pyrimidine bases (T or U) when piperidine, instead of DBU, is used to remove the Fmoc groups.

引用

页码：2195 / 2198

页数：4

共 15 条

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