SYNTHESIS OF AMINO-ACID DERIVATIVES AND DIPEPTIDES WITH AN ORIGINAL PEPTIDASE ENZYME

被引：0

作者：

AURIOL, D ^{[1
]}

PAUL, F ^{[1
]}

YOSHPE, I ^{[1
]}

GRIPON, JC ^{[1
]}

MONSAN, P ^{[1
]}

机构：

[1] INRA,RECH LAITIERES STN,F-78352 JOUY EN JOSAS,FRANCE

来源：

BIOMEDICA BIOCHIMICA ACTA | 1991年 / 50卷 / 10-11期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A peptidase from the non pathogenic Staphylococcus sp. strain BEC 299 was purified to a final specific activity of 84,400 U/mg protein. Its molecular weight is 450 kDa and optimum pH 10.0. This enzyme catalyzes the synthesis of dipeptides (aspartame) and alpha-amino acid derivatives (N-L-malyl-L-tyrosine ethyl ester). The influence of cosolvents and pH on dipeptides and alpha-amino acid derivative synthesis is described. Finally, we detail the use of the peptidase as a reagent in protease-catalyzed peptide synthesis.

引用

页码：S163 / S168

页数：6

共 5 条

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[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1985, 24 (02): : 85 - 93
[5] PAUL F, 1988, ANN NY ACAD SCI, V542, P351

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