SEX IDENTIFICATION BY POLYMERASE CHAIN-REACTION USING X-Y HOMOLOGOUS PRIMER

被引：156

作者：

NAKAHORI, Y

HAMANO, K

IWAYA, M

NAKAGOME, Y

机构：

[1] NATL CHILDRENS MED RES CTR,DEPT CONGENITAL ABNORMALITIES RES,3-35-31 TAISHIDO,SETAGAYA KU,TOKYO 154,JAPAN

[2] NATL CHILDRENS MED RES CTR,DEPT INFECT DIS RES,SETAGAYA KU,TOKYO,JAPAN

来源：

AMERICAN JOURNAL OF MEDICAL GENETICS | 1991年 / 39卷 / 04期

关键词：

PRIMERS; PCR; X-SPECIFIC BAND; Y-SPECIFIC BAND;

D O I：

10.1002/ajmg.1320390420

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A method of sex identification using the polymerase chain reaction technique is described. Using a pair of nucleotide primers from an X-Y homologous region, both the X and the Y sequences can be amplified simultaneously, and more importantly, they result in fragments of different lengths. The success of the procedure is therefore monitored by the presence of a X-specific band while sex is identified by the presence or absence of a Y-specific band.

引用

页码：472 / 473

页数：2

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