CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF GC, THE VITAMIN D-BINDING PROTEIN IN SERUM

被引：8

作者：

VOGELAAR, NJ ^{[1
]}

LINDBERG, U ^{[1
]}

SCHUTT, CE ^{[1
]}

机构：

[1] UNIV STOCKHOLM,DEPT ZOOL CELL BIOL,S-10691 STOCKHOLM,SWEDEN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 220卷 / 03期

关键词：

GC; CRYSTALLIZATION; VITAMIN D-BINDING (PROTEIN); X-RAY DIFFRACTION; IGG-BINDING (PROTEIN);

D O I：

10.1016/0022-2836(91)90097-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The vitamin D-binding protein, Gc, was purified from human serum and crystallized using the hanging-drop method. The best crystals were grown from 28% polyethylene glycol 400 in 50 mm-sodium acetate at pH 4·8. These crystals diffract to 3.4 Å and the observed diffraction is consistent with orthorhombic space groups P41 and P43. The unit cell parameters were determined to be a = b = 135·5 A ̊ and c = 75·6 A ̊. © 1991.

引用

页码：545 / 547

页数：3

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