Calcium/calmodulin-regulated receptor-like kinase CRLK1 interacts with MEKK1 in plants

被引：13
作者：
Yang, Tianbao [1 ,2 ]
Ali, Gul Shad [3 ,4 ]
Yang, Lihua [1 ,2 ]
Du, Liqun [1 ,2 ]
Reddy, A. S. N. [3 ,4 ]
Poovaiah, B. W. [1 ,2 ]
机构：
[1] Washington State Univ, Ctr Integrated Biotechnol, Pullman, WA 99164 USA
[2] Washington State Univ, Dept Hort, Pullman, WA 99164 USA
[3] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA
[4] Colorado State Univ, Program Mol Plant Biol, Ft Collins, CO 80523 USA
来源：
PLANT SIGNALING & BEHAVIOR | 2010年 / 5卷 / 08期
基金：
美国食品与农业研究所; 美国国家科学基金会;
关键词：
calcium; calmodulin; cold stress; MAPK; Arabidopsis; protein phosphorylation;
D O I：
10.4161/psb.5.8.12225
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recently we reported that CRLK1, a novel calcium/calmodulin-regulated receptor-like kinase plays an important role in regulating plant cold tolerance. Calcium/calmodulin binds to CRLK1 and upregulates its activity. Gene knockout and complementation studies revealed that CRLK1 is a positive regulator of plant response to chilling and freezing temperatures. Here we show that MEKK1, a member of MAP kinase kinase kinase family, interacts with CRLK1 both in vitro and in planta. The cold triggered MAP kinase activation in wild-type plants was abolished in crlk1 knockout mutants. Similarly, the cold induced expression levels of genes involved in MAP kinase signaling are also altered in crlk1 mutants. These results suggest that calcium/calmodulin-regulated CRLK1 modulates cold acclimation through MAP kinase cascade in plants.
引用
收藏
页码：991 / 994
页数：4
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