SEQUENCE OF THE GENE ENCODING THE 16S RIBOSOMAL-RNA OF THE BEER SPOILAGE ORGANISM MEGASPHAERA-CEREVISIAE

被引:10
|
作者
DOYLE, LM
MCINERNEY, JO
MOONEY, J
POWELL, R
HAIKARA, A
MORAN, AP
机构
[1] UNIV COLL GALWAY,DEPT MICROBIOL,GALWAY,IRELAND
[2] TECH RES CTR FINLAND,BIOTECHNOL & FOOD RES LAB,ESPOO,FINLAND
来源
JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1995年 / 15卷 / 02期
关键词
MEGASPHAERA CEREVISIAE; 16S RIBOSOMAL-RNA; DNA PROBE; BEER SPOILAGE; PHYLOGENY;
D O I
10.1007/BF01569801
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The 16S ribosomal RNA gene from the beer-spoilage organism, Megasphaera cerevisiae was polymerase chain reaction (PCR)-amplified and sequenced. Analysis confirmed the phylogenetic position of M. cerevisiae as a sister taxon of Megasphaera elsdenii, within the obligately anaerobic, Gram-negative cocci. The sequence obtained should facilitate the development of DNA probes for early detection of this spoilage organism,
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页码:67 / 70
页数:4
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