STRUCTURAL AND FUNCTIONAL CONSERVATION OF 2 HUMAN HOMOLOGS OF THE YEAST DNA-REPAIR GENE RAD6

被引：226
作者：
KOKEN, MHM
REYNOLDS, P
JASPERSDEKKER, I
PRAKASH, L
PRAKASH, S
BOOTSMA, D
HOEIJMAKERS, JHJ
机构：
[1] UNIV ROCHESTER,DEPT BIOL,RIVER CAMPUS STN,ROCHESTER,NY 14627
[2] ERASMUS UNIV,DEPT CELL BIOL & GENET,3000 DR ROTTERDAM,NETHERLANDS
[3] UNIV ROCHESTER,SCH MED,DEPT BIOPHYS,ROCHESTER,NY 14627
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 20期
关键词：
UBIQUITIN CONJUGATION; E2; ENZYME; DNA DAMAGE; UV MUTAGENESIS; SPORULATION;
D O I：
10.1073/pnas.88.20.8865
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other and almost-equal-to 70% and almost-equal-to 85% overall identity with the homologs from yeasts (S. cerevisiae and Schizosaccharomyces pombe) and Drosophila melanogaster, respectively. Neither of the human RAD6 homologs possesses the acidic C-terminal sequence present in the S. cerevisiae RAD6 protein. Genetic complementation experiments reveal that HHR6A as well as HHR6B can carry out the DNA repair and mutagenesis functions of RAD6 in S. cerevisiae rad6-DELTA mutants.
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页码：8865 / 8869
页数：5
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