TELOMERIC STRUCTURES IN A LINEAR MITOCHONDRIAL PLASMID FROM PHYSARUM-POLYCEPHALUM

被引:12
作者
TAKANO, H
KAWANO, S
KUROIWA, T
机构
[1] Department of Biology, Faculty of Science, University of Tokyo, Tokyo, 113, Hongo
关键词
PHYSARUM-POLYCEPHALUM; MITOCHONDRIAL PLASMID; LINEARITY; TELOMERIC STRUCTURE;
D O I
10.1007/BF00318521
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A mitochondrial plasmid was isolated from Physarum polycephalum and characterized by restriction mapping. Cloned fragments of the plasmid were assembled and used to construct a restriction map. This plasmid was a linear molecule with telomeric structures at each end. Southern hybridization with the ends of the plasmid as probes revealed that the plasmid included repeating units at both ends, with each unit being approximately 125 bp in length. The most extensive array of repeats consisted of at least 17 repetitions of the 125-bp unit. The sensitivity of these repeats to Bal31 exonuclease confirmed that they were at, or very near to, the ends of the plasmid. From the extent of the repetitions, the size of the plasmid was estimated to vary from 13.3 kbp to more than 18.3 kbp.
引用
收藏
页码:315 / 317
页数:3
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