THE PSI-E SUBUNIT OF PHOTOSYSTEM-I BINDS FERREDOXIN-NADP+ OXIDOREDUCTASE

被引：104
作者：
ANDERSEN, B
SCHELLER, HV
MOLLER, BL
机构：
[1] Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, DK-1871 Frederiksberg C, Copenhagen
来源：
FEBS LETTERS | 1992年 / 311卷 / 02期
关键词：
PHOTOSYSTEM-I; FERREDOXIN-NADP+ OXIDOREDUCTASE; CROSS-LINKING; PSI-E; FNR-BINDING PROTEIN;
D O I：
10.1016/0014-5793(92)81391-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A photosystem I complex containing the polypeptides PSI-A to PSI-L, light-harvesting complex I and ferredoxin:NADP+ oxidoreductase has been isolated from barley using the non-ionic detergent n-decyl-beta-D-maltopyranoside. The ratio between bound ferredoxin:NADP+ oxidoreductase and P700 is 0.4 +/- 0.2. The complex is highly active in catalyzing light-induced transfer of electrons from plastocyanin to NADP+ at rates of 280+/-150 and 1800 +/- 800 mumol NADPH/(mg chl.h), without and in the presence of saturating amounts of exogenously added ferredoxin:NADP+ oxidoreductase, respectively. Endogenously bound ferredoxin:NADP+ oxidoreductase interacts with the PSI-E subunit as demonstrated by cross-linking experiments using two different types of cross-linkers and identification of the products by Western blotting and the use of monospecific antibodies.
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页码：169 / 173
页数：5
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