IMMOBILIZATION STABILIZATION ON DIFFERENT HYDROXYLIC SUPPORTS OF LIPASE FROM CANDIDA-RUGOSA

被引：27

作者：

ARROYO, M ^{[1
]}

MORENO, JM ^{[1
]}

SINISTERRA, JV ^{[1
]}

机构：

[1] UNIV COMPLUTENSE,FAC PHARM,DEPT ORGAN & PHARMACEUT CHEM,E-28040 MADRID,SPAIN

来源：

JOURNAL OF MOLECULAR CATALYSIS | 1993年 / 83卷 / 1-2期

关键词：

CANDIDA-RUGOSA; ENZYME IMMOBILIZATION; LIPASE;

D O I：

10.1016/0304-5102(93)87024-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Lipase from Candida rugosa has been covalently immobilized on tosyl activated matrix (agarose and corn cob) and optimum immobilisation conditions determined. The immobilized derivatives exhibited greater residual activity than the ones reported previously. Studies on the activity and stability of the different insolubilized derivatives prepared showed that the enzymatic derivative immobilized on small corn cob is resistant to inactivation by temperature, at 50-degrees-C. This derivative was 100 times more stable than its soluble counterpart. The insolubilized derivatives are more active and stable at higher temperatures (> 35-degrees-C) than soluble enzyme. Lipase activity (using olive oil emulsion) and esterase activity (using p-nitrophenyl butyrate) have been determined. Kinetic studies have been carried out with soluble and immobilized derivatives. The influence of Na (I) and Ca (II) on the lipase and esterase activities is discussed.

引用

页码：261 / 271

页数：11

共 28 条

[1]

Alcantara A, 1988, Ann N Y Acad Sci, V542, P1, DOI 10.1111/j.1749-6632.1988.tb25801.x

[2]

ALCANTARA A, 1990, APPL BIOCHEM BIOTECH, V29, P297

[3] POSITIONAL AND CHAIN SPECIFICITIES OF CANDIDA-CYLINDRACEA LIPASE [J].

BENZONANA, G ;

ESPOSITO, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 231 (01) :15-+

[4] COMPARISON OF DIFFERENT STRATEGIES FOR THE LIPASE-CATALYZED PREPARATIVE RESOLUTION OF RACEMIC ACIDS AND ALCOHOLS - ASYMMETRIC HYDROLYSIS, ESTERIFICATION, AND TRANS-ESTERIFICATION [J].

CAMBOU, B ;

KLIBANOV, AM .

BIOTECHNOLOGY AND BIOENGINEERING, 1984, 26 (12) :1449-1454

[5] ENZYMATIC-SYNTHESIS OF ESTERS USING AN IMMOBILIZED LIPASE [J].

CARTA, G ;

GAINER, JL ;

BENTON, AH .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (11) :1004-1009

[6] ACTION OF PANCREATIC LIPASE ON AGGREGATED GLYCERIDE MOLECULES IN AN ISOTROPIC SYSTEM [J].

ENTRESSANGLES, B ;

DESNUELLE, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 159 (02) :285-+

[7] ACTIVITY OF LIPASE IN WATER-IN-OIL MICROEMULSIONS [J].

FLETCHER, PDI ;

ROBINSON, BH ;

FREEDMAN, RB ;

OLDFIELD, C .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1985, 81 :2667-2679

[8] ANIONIC LIPID HEADGROUPS AS A PROTON-CONDUCTING PATHWAY ALONG THE SURFACE OF MEMBRANES - A HYPOTHESIS [J].

HAINES, TH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :160-164

[9] CHARACTERISTICS OF LIPASE-CATALYZED HYDROLYSIS OF OLIVE OIL IN AOT-ISOOCTANE REVERSED MICELLES [J].

HAN, D ;

RHEE, JS .

BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (08) :1250-1255

[10] LATERAL DISTRIBUTION OF NEGATIVELY CHARGED LIPIDS IN LECITHIN MEMBRANES - CLUSTERING OF FATTY-ACIDS [J].

HAUSER, H ;

GUYER, W ;

HOWELL, K .

BIOCHEMISTRY, 1979, 18 (15) :3285-3291

← 1 2 3 →