INTERPHASE MOLECULAR CYTOGENETICS OF EWINGS-SARCOMA AND PERIPHERAL NEUROEPITHELIOMA T(1122) WITH FLANKING AND OVERLAPPING COSMID PROBES

被引：62

作者：

DESMAZE, C ^{[1
]}

ZUCMAN, J ^{[1
]}

DELATTRE, O ^{[1
]}

MELOT, T ^{[1
]}

THOMAS, G ^{[1
]}

AURIAS, A ^{[1
]}

机构：

[1] INST CURIE,INSERM,CJF 9201,GENET TUMEURS LAB,F-75231 PARIS 05,FRANCE

来源：

CANCER GENETICS AND CYTOGENETICS | 1994年 / 74卷 / 01期

关键词：

D O I：

10.1016/0165-4608(94)90022-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The translocation, t(11;22)(q24;q12), recurrently observed in Ewing's sarcoma and in peripheral neuroepithelioma has been recently cloned. The analysis of a series of ES/PNE has revealed that the chromosome 22 breakpoints are clustered in a small region of 7 kb, called EWSR1, and that those on chromosome 11 are spread over a larger region of 40-50 kb, called EWSR2. Cosmids from loci flanking or overlapping these two regions have been obtained. We demonstrate here that fluorescence in situ hybridization (FISH) with these cosmids allows the localization of the two breakpoints with a 10-kb resolution and leads to a rapid and reliable ES/PNE diagnosis.

引用

页码：13 / 18

页数：6

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