REGULATION OF ACETYLCHOLINE-RECEPTOR DESENSITIZATION IN MOUSE MYOTUBES BY CYTOSOLIC CYCLIC-AMP

被引：5
作者：
GIOVANNELLI, A
FARINI, D
GAUZZI, MC
ALEMA, S
EUSEBI, F
机构：
[1] UNIV LAQUILA,DIPARTIMENTO MED SPERIMENTALE,COLLEMAGGIO,I-67100 LAQUILA,ITALY
[2] UNIV ROME LA SAPIENZA,INST PHYS CHIM RAYONNEMENTS,I-00185 ROME,ITALY
[3] CNR,IST BIOL CELLULARE,I-00196 ROME,ITALY
来源：
CELLULAR SIGNALLING | 1990年 / 2卷 / 04期
关键词：
cAMP; CGRP; desensitization; forskolin; myotube; nAChR;
D O I：
10.1016/0898-6568(90)90064-H
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Whole-Cell currents activated by bath applications of acetylcholine (ACh) (0-30 μM) were recorded from patch-clamped myotubes of the mouse C2 cell line. Increasing concentrations of forskolin caused a dose-dependent fast decay of ACh-activated currents as compared to the long-lasting ACh-currents in control cells. The forskolin-induced modulation of nicotinic ACh receptor (nAChR) desensitization was proportional to the drug-induced elevation in the cyclic AMP (cAMP) cellular. Furthermore, an increase in the rate of decay of the ACh-current response, which paralleled an elevation in cAMP cellular content, was caused by treatment with a calcitonin gene-related peptide (1 μM), 8-Br-cAMP) (0.5 mM), or by loading the myotubes with cAMP. These results therefore indicate that the desensitization of nAChR is a cAMP-related process in C2-myotubes. © 1990.
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收藏
页码：347 / 352
页数：6
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