SAK, A MURINE PROTEIN-SERINE/THREONINE KINASE THAT IS RELATED TO THE DROSOPHILA-POLO KINASE AND INVOLVED IN CELL-PROLIFERATION

被引：89

作者：

FODE, C ^{[1
]}

MOTRO, B ^{[1
]}

YOUSEFI, S ^{[1
]}

HEFFERNAN, M ^{[1
]}

DENNIS, JW ^{[1
]}

机构：

[1] UNIV TORONTO, DEPT MED GENET, TORONTO M5S 1A8, ON, CANADA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 14期

关键词：

D O I：

10.1073/pnas.91.14.6388

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated murine cDNAs encoding two isoforms of a putative protein-serine/threonine kinase, designated Sak-a and Sak-b, which differ in their noncatalytic C-terminal ends. The kinase domain of Sak is related to the catalyic domains of the Drosophila polo, Saccharomyces cerevisiae CDC5, and murine Snk and Plk kinases, a family of proteins for which a role in controlling cell proliferation has been established (polo, CDC5) or implicated (Snk, Plk). Northern and in situ RNA analyses of Sak gene expression in mouse embryos and adult tissues revealed that expression was associated with mitotic and meiotic cell division. In addition, during embryogenesis, Sak expression was prominent in the respiratory and olfactory mucosa. The pattern of Sak expression and its sequence homology with the polo gene family suggest that the Sak kinase may play a role in cell proliferation. In support of this, cell growth was suppressed by expression of a Sak-a antisense fragment in CHO cells.

引用

页码：6388 / 6392

页数：5

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