MEMBRANE-PROTEIN SPANNING SEGMENTS AS EXPORT SIGNALS

被引：65

作者：

CALAMIA, J

MANOIL, C

机构：

[1] Department of Genetics, SK-50 University of Washington, Seattle

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 224卷 / 03期

关键词：

MEMBRANE PROTEIN; LAC PERMEASE; ALKALINE PHOSPHATASE; GENE FUSION;

D O I：

10.1016/0022-2836(92)90542-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli lac permease is a polytopic integral membrane protein with six translocated (periplasmic) domains. Individual N-terminal cytoplasmic regions and membrane-spanning segments adjacent to each of the periplasmic domains acted as export signals for an attached sensor protein (alkaline phosphatase). However, the export activity of one of the spanning segments was considerably lower than that of the others, and was limited by the presence of a positively charged residue (Arg302). These observations are compatible with models of membrane protein insertion in which hydrophilic domains are translocated independently. However, the results suggest that efficient translocation may sometimes require interaction between individual spanning segments. © 1992.

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页码：539 / 543

页数：5

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