DETECTION OF APC REGION-SPECIFIC SIGNALS BY NONISOTOPIC CHROMOSOMAL INSITU SUPPRESSION (CISS)-HYBRIDIZATION USING A MICRODISSECTION LIBRARY AS A PROBE

被引：0

作者：

TRAUTMANN, U ^{[1
]}

LEUTERITZ, G ^{[1
]}

SENGER, G ^{[1
]}

CLAUSSEN, U ^{[1
]}

BALLHAUSEN, WG ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG,INST HUMANGENET,SCHWABACHANLAGE 10,W-8520 ERLANGEN,GERMANY

来源：

HUMAN GENETICS | 1991年 / 87卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The chromosome region 5q22 harbouring the putative gene associated with adenomatous polyposis coli (APC) was microdissected and microcloned from GTG-banded human metaphase chromosomes. In order to determine the precise regional localization of the microdissected material, we used polymerase chain reaction amplified microclones as a bulk-probe in nonradioactive chromosomal in situ suppression hybridization of human metaphase spreads. Specific in situ hybridization signals were obtained on the long arm of chromosome 5 in accordance with the chromosomal region excised for the cloning procedure. The application of this detection system should provide a rapid and powerful tool for analyzing patients with translocations or microdeletions of a given chromosome region.

引用

页码：495 / 497

页数：3

共 8 条

[1]

[Anonymous], 1985, INT SYSTEM HUMAN CYT

[2] LOCALIZATION OF THE GENE FOR FAMILIAL ADENOMATOUS POLYPOSIS ON CHROMOSOME-5 [J].

BODMER, WF ;

BAILEY, CJ ;

BODMER, J ;

BUSSEY, HJR ;

ELLIS, A ;

GORMAN, P ;

LUCIBELLO, FC ;

MURDAY, VA ;

RIDER, SH ;

SCAMBLER, P ;

SHEER, D ;

SOLOMON, E ;

SPURR, NK .

NATURE, 1987, 328 (6131) :614-616

[3]

JAUCH A, 1990, HUM GENET, V85, P145

[4] THE GENE FOR FAMILIAL POLYPOSIS-COLI MAPS TO THE LONG ARM OF CHROMOSOME-5 [J].

LEPPERT, M ;

DOBBS, M ;

SCAMBLER, P ;

OCONNELL, P ;

NAKAMURA, Y ;

STAUFFER, D ;

WOODWARD, S ;

BURT, R ;

HUGHES, J ;

GARDNER, E ;

LATHROP, M ;

WASMUTH, J ;

LALOUEL, JM ;

WHITE, R .

SCIENCE, 1987, 238 (4832) :1411-1413

[5] DELINEATION OF INDIVIDUAL HUMAN-CHROMOSOMES IN METAPHASE AND INTERPHASE CELLS BY INSITU SUPPRESSION HYBRIDIZATION USING RECOMBINANT DNA LIBRARIES [J].

LICHTER, P ;

CREMER, T ;

BORDEN, J ;

MANUELIDIS, L ;

WARD, DC .

HUMAN GENETICS, 1988, 80 (03) :224-234

[6]

LUDECKE HJ, 1990, HUM GENET, V84, P512

[7] CLONING DEFINED REGIONS OF THE HUMAN GENOME BY MICRODISSECTION OF BANDED CHROMOSOMES AND ENZYMATIC AMPLIFICATION [J].

LUDECKE, HJ ;

SENGER, G ;

CLAUSSEN, U ;

HORSTHEMKE, B .

NATURE, 1989, 338 (6213) :348-350

[8]

SENGER G, 1990, HUM GENET, V84, P507

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