FLUOROMETRIC DETECTION AFTER LIQUID-CHROMATOGRAPHIC SEPARATION OF BENZOPHENANTHRIDINE ALKALOIDS OBTAINED FROM CULTURED-CELLS

被引：9

作者：

CHAURET, N

ARCHAMBAULT, J

机构：

[1] Biotechnology Research Institute, National Research Council Canada, Montréal, H4P 2R2

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 249卷 / 01期

关键词：

LIQUID CHROMATOGRAPHY; FLOW SYSTEM; ALKALOIDS; BENZOPHENANTHRIDINE;

D O I：

10.1016/0003-2670(91)87027-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The detection by fluorimetry after liquid chromatographic (LC) separation of benzophenanthridine alkaloids extracted from Papaveraceae sp. cell culture samples was characterized and optimized. Maximum fluorescence was observed at 225 nm excitation and 410 nm emission wavelengths for most alkaloids studied (chelirubine, sanguinarine, chelerythrine, chelilutine, sanguilutine, sanguirubine and norsanguinarine). Under these conditions, the detection limits varied from 0.1 to 0.7 ng. The low detection limits and selectivity of fluorescence detection allowed alkaloids to be quantified by LC in crude cell extracts and medium samples obtained from different cultures without any pretreatment. The relative standard deviation of the LC method varied from 1.0 to 2.6% for the alkaloids assessed.

引用

页码：231 / 234

页数：4

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