CAPILLARY ELECTROKINETIC CHROMATOGRAPHY WITH A SUSPENSION OF CHROMATOGRAPHIC PARTICLES

The use of a suspension of chromatographic particles as a pseudo-stationary phase in capillary electrokinetic chromatography is demonstrated. The separation of nine phenol derivatives is presented to demonstrate the influence of the particles. Reversed-phase particles with a diameter of 1.5 mu m were chosen. These particles were coated with a surfactant to form a stable suspension. The capacity factor of the chromatographic separation can be varied by changing the particle concentration, independent of other parameters. To avoid light scattering at the particles, a discontinuous set-up was developed.