CLONING AND EXPRESSION OF A PORCINE UDP-GALNAC - POLYPEPTIDE N-ACETYLGALACTOSAMINYL TRANSFERASE

被引：15

作者：

YOSHIDA, A

HARA, T

IKENAGA, H

TAKEUCHI, M

机构：

[1] Central Laboratories for Key Technology, Kirin Brewery Co., Ltd, Yokohama, 236, Fukuura, Kanazawa-ku

来源：

GLYCOCONJUGATE JOURNAL | 1995年 / 12卷 / 06期

关键词：

UDP-N-ACETYLGALACTOSAMINE; POLYPEPTIDE N-ACETYL GALACTOSAMINYL TRANSFERASE; GLYCOSYLTRANSFERASE; O-LINKED SUGARS; MUCIN; PORCINE;

D O I：

10.1007/BF00731244

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By employing a bovine UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyl transferase (O-GalNAc transferase) cDNA as a probe, we isolated four overlapping cDNAs from a porcine lung cDNA library. Both the nucleotide sequence of the porcine cDNA and the predicted primary structure of the protein (559 amino acids) proved to be very similar to those of the bovine enzyme (95% and 99% identity, respectively). Transient expression of the clone in COS-7 cells, followed by enzymatic activity assays, demonstrated that this cDNA sequence encodes a porcine O-GalNAc transferase. The intracellular O-GalNAc transferase activity was increased approximately 100-fold by transfecting cells with the porcine cDNA.

引用

页码：824 / 828

页数：5

共 23 条

[1]

DOHI DF, 1993, J BIOL CHEM, V268, P10133

[2] 2 ENZYMES INVOLVED IN THE SYNTHESIS OF O-LINKED OLIGOSACCHARIDES ARE LOCALIZED ON MEMBRANES OF DIFFERENT DENSITIES IN MOUSE LYMPHOMA BW5147-CELLS [J].