SPECIFIC EFFECTS OF MODIFIERS IN SUBCRITICAL FLUID CHROMATOGRAPHY OF CAROTENOID-PIGMENTS

被引：22

作者：

LESELLIER, E ^{[1
]}

KRSTULOVIC, AM ^{[1
]}

TCHAPLA, A ^{[1
]}

机构：

[1] SYNTHELABO RECH,LERS,F-92360 MEUDON,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 641卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)83467-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of subcritical fluid chromatography with filled columns for the analysis of carotenoid pigments affords a threefold reduction in analysis times with respect to HPLC. The addition of modifiers to CO2 increases the solubility of pigments in the mobile phase. This paper reports the influence of modifiers on the separation of carotenoids in terms of specific interactions, such as hydrogen bonding and pi-pi interactions between the solvent and the solute. The understanding of these interactions allowed the optimization of the separation of seven carotenoids and some of their cis isomers; this separation required less than 20 min.

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页码：137 / 145

页数：9

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