Multiplex PCR for Rapid Diagnosis of Gastrointestinal Tuberculosis

被引：26

作者：

Sharma, Kusum ^{[1
,2
]}

Sinha, Saroj Kant ^{[2
]}

Sharma, Aman ^{[3
]}

Nada, Ritambra ^{[4
]}

Prasad, Kaushal K. ^{[2
]}

Goyal, Kapil ^{[1
]}

Rana, Surinder Singh ^{[2
]}

Bhasin, Deepak Kumar ^{[2
]}

Sharma, Meera ^{[1
]}

机构：

[1] Post Grad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh, India

[2] Post Grad Inst Med Educ & Res, Dept Gastroenterol, Chandigarh, India

[3] Post Grad Inst Med Educ & Res, Dept Internal Med, Chandigarh, India

[4] Post Grad Inst Med Educ & Res, Dept Pathol, Chandigarh, India

来源：

JOURNAL OF GLOBAL INFECTIOUS DISEASES | 2013年 / 5卷 / 02期

关键词：

Gastrointestinal tract; Multiplex PCR; Tuberculosis;

D O I：

10.4103/0974-777X.112272

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance. Aim: To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB. Materials and Methods: MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 Non GITB (control group) patients. Results: MPB64 PCR had sensitivity and specificity of 90% and 100% for confirmed GITB cases. In 29 clinically diagnosed but unconfirmed GITB cases, MPCR was positive in 72.41%. MPCR was negative in all control group patients. The overall sensitivity and specificity of microscopy, culture, histopathology and MPCR was 5%, 2% 20% and 77.5% and 100%, 100%, 100% and 100% respectively. Conclusion: MPCR has good sensitivity and specificity in diagnosing gastrointestinal tuberculosis.

引用

页码：49 / 53

页数：5

共 25 条

[1] Clinical, colonoscopic, and histological profile of colonic tuberculosis in a tertiary hospital [J].

Alvares, JF ;

Devarbhavi, H ;

Makhija, P ;

Rao, S ;

Kottoor, R .

ENDOSCOPY, 2005, 37 (04) :351-356

[2]

Amarapurkar D N, 2004, J Assoc Physicians India, V52, P863

[3]

BHASIN DK, 1991, LANCET, V338, P184

[4] Diagnosis of tuberculous meningitis: clinical and laboratory parameters [J].

Bhigjee, Ahmed Iqbal ;

Padayachee, Rivashnee ;

Paruk, Hoosain ;

Hallwirth-Pillay, Kumari Devi ;

Marais, Suzaan ;

Connoly, Cathy .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2007, 11 (04) :348-354

[5]

Chauhan DS, 2007, INDIAN J MED RES, V125, P577

[6] Crohn's Disease in India: A Multicenter Study from a Country Where Tuberculosis Is Endemic [J].

Das, Kshaunish ;

Ghoshal, Uday C. ;

Dhali, Gopal K. ;

Benjamin, Jaya ;

Ahuja, Vineet ;

Makharia, Govind K. .

DIGESTIVE DISEASES AND SCIENCES, 2009, 54 (05) :1099-1107

[7] Improved diagnosis of central nervous system tuberculosis by MPB64-target PCR [J].

Dil-Afroze ;

Mir, Abdul Waheed ;

Kirmani, Altaf ;

Shakeel-ul-Rehman ;

Eachkoti, Rafiqa ;

Siddiqi, Mushtaq A. .

BRAZILIAN JOURNAL OF MICROBIOLOGY, 2008, 39 (02) :209-213

[8]

Gan HT, 2002, AM J GASTROENTEROL, V97, P1446, DOI 10.1111/j.1572-0241.2002.05686.x

[9] Histopathology and TB-PCR kit analysis in differentiating the diagnosis of intestinal tuberculosis and Crohn's disease [J].

Jin, Xian Ji ;

Kim, Joon Mee ;

Kim, Hyung Kil ;

Kim, Lucia ;

Choi, Suk Jin ;

Park, In Suh ;

Han, Jee Young ;

Chu, Young Chae ;

Song, Ju Young ;

Kwon, Kye Sook ;

Kim, Eun Joo .

WORLD JOURNAL OF GASTROENTEROLOGY, 2010, 16 (20) :2496-2503

[10] Abdominal tuberculosis [J].

Kapoor, VK .

POSTGRADUATE MEDICAL JOURNAL, 1998, 74 (874) :459-467

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