Aim: An attempt was made to assess the effect of pure enzyme combinations with the objective of formulating customized enzyme mixtures based on sugar release when subjected to two-stage in vitro digestion assay. Materials and Methods: A two-stage in vitro digestibility assay was carried out for commonly used feed ingredients for poultry viz., maize, soy bean meal, sunflower cake, and de-oiled rice bran supplemented with three concentrations of xylanase (5000; 7500 and 10000 IU/kg), cellulase (50; 100 and 400 IU/kg) and beta-D-glucanase (100; 200 and 400 IU/kg) were used to formulate various NSP enzymes combinations. In total 27 NSP enzyme combinations (3x3x3) were formulated and the sugar released due to NSP digestion was quantified by phenol sulphuric acid method. Results: The total sugar release was significantly (P<0.05) higher with supplementation of various enzymes combinations for maize, sunflower cake and de-oiled rice bran where as no significant (P<0.05) interaction of various NSP enzymes combinations was observed for soy bean meal. The NSP digestibility was highest in combination (xylanase-5000, cellulase-50 and beta-D-glucanase-400 IU/kg), (xylanase-10000, cellulase-50 and beta-D-glucanase-200 IU/kg) and (xylanase-7500, cellulase100 and beta-D-glucanase-100 IU/kg) for maize, sunflower cake and de-oiled rice bran respectively. In case of sunflower cake, significant (P<0.01) three way interaction was observed among the xylanase, cellulose, and beta-D-glucanase enzymes and the two-way interactions between the enzymes were also significant (P<0.01). Conclusion: It is concluded that 'n' number of non-starch Polysaccharide enzymes combinations can be screened for their efficiency to digest non-starch Polysaccharides present in various feed ingredients commonly used in poultry rations by employing two-stage in vitro digestibility assay as a tool.
