Expressions of 11beta-Hydroxysteroid Dehydrogenase Types 1 and 2 in Human Colorectal Cancer

被引：0
作者：
Wang Ji-rong [1 ]
Xu Hong-xia [2 ]
机构：
[1] Nanjing Med Univ, Affiliated Hosp 2, Dept Oncol, Nanjing 210011, Jiangsu, Peoples R China
[2] Jiangsu Tumor Hosp, Dept Internal Med, Nanjing 210011, Jiangsu, Peoples R China
来源：
JOURNAL OF INTERNATIONAL TRANSLATIONAL MEDICINE | 2014年 / 2卷 / 03期
关键词：
Colorectal cancer; Human; 11; beta-HSD1; gene; beta-HSD2;
D O I：
10.11910/2227-6394.2014.02.03.11
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Objective: To observe the expressions of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in colorectal cancer. Methods: Neoplastic tissues and autologous non-neoplastic tissues were taken from 12 patients with colorectal cancer immediately after the operations. The mRNA expressions of 11betahydroxysteroid dehydrogenase type 1 (11 beta-HSD1) and 11beta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2) were detected by reverse transcription polymerase chain reaction (RT-PCR). The protein expressions of 11 beta-HSD1 and 11 beta-HSD2 were detected by Western-blot assay. Results: Both isoforms of 11 beta-HSD expressed in the colon adenocarcinoma, but their expressions were not identical in neoplastic and non-neoplastic tissues. There was a significant decrease of 11 beta-HSD2 mRNA abundance and protein abundance in neoplastic tissue. 11 beta-HSD1 protein and mRNA abundance were not significantly different from that in control tissue. Conclusions: Neoplastic transformation is associated with the decreases of 11 beta-HSD2 mRNA and protein.
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页码：403 / 407
页数：5
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