LOCALIZATION OF PHOSPHOPROTEIN C23 TO NUCLEOLAR STRUCTURES AND TO THE NUCLEOLUS ORGANIZER REGIONS

被引：172

作者：

LISCHWE, MA ^{[1
]}

RICHARDS, RL ^{[1
]}

BUSCH, RK ^{[1
]}

BUSCH, H ^{[1
]}

机构：

[1] BAYLOR COLL MED, DEPT PHARMACOL, HOUSTON, TX 77030 USA

来源：

EXPERIMENTAL CELL RESEARCH | 1981年 / 136卷 / 01期

关键词：

D O I：

10.1016/0014-4827(81)90041-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

After extraction of [rat V,S,-73] Novikoff hepatoma nucleoli with 4 M urea/3 M LiCl, phosphoprotein C23 was isolated by DEAE-cellulose and Bio-Rad AG3-X4A column chromatography. Immunization of rabbits with the highly purified protein C23 resulted in the production of a specific antibody as determined by Ouchterlony diffusion analysis. When the immunoperoxidase method was used to localize protein C23 in cells [human epidermoid carcinoma KB cell, laryngeal carcinoma HEp-2 cell, mouse fibroblast, colon tumor C856 B cell, mammary tumor T-9734-126 cell, human liver cell, thioacetamide rat liver extract, regenerating mouse liver cell and mouse fibroblast 3T3 cells], it was found in fibrillar centers (nucleolonemas) in nucleoli. Protein C23 was present on the nucleolus organizer regions (NOR) of metaphase chromosomes.

引用

页码：101 / 109

页数：9

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