MUTUALLY EXCLUSIVE EXON SPLICING OF THE CARDIAC CALCIUM-CHANNEL ALPHA-1 SUBUNIT GENE GENERATES DEVELOPMENTALLY REGULATED ISOFORMS IN THE RAT-HEART

被引：92

作者：

DIEBOLD, RJ ^{[1
]}

KOCH, WJ ^{[1
]}

ELLINOR, PT ^{[1
]}

WANG, JJ ^{[1
]}

MUTHUCHAMY, M ^{[1
]}

WIECZOREK, DF ^{[1
]}

SCHWARTZ, A ^{[1
]}

机构：

[1] UNIV CINCINNATI,COLL MED,DEPT MOLEC GENET BIOCHEM & MICROBIOL,CINCINNATI,OH 45229

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 04期

关键词：

D O I：

10.1073/pnas.89.4.1497

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Several clones were isolated from a rat genomic library in order to further characterize a region of variability within the third membrane-spanning region of the fourth motif (IVS3) of the L-type voltage-dependent calcium channel. We report here that this diversity arises from alternative splicing of a primary transcript containing a single pair of adjacent exons each encoding a unique sequence for the IVS3 region. Definitive proof of a mutually exclusive splicing mechanism was obtained by genomic mapping of flanking upstream and downstream exons and by extensive sequence analysis of the relevant exon/intron boundaries. S1 nuclease protection experiments revealed that both variant forms of the IVS3 were equally expressed in newborn and fetal rat heart, whereas only a single isoform predominated in adult rat heart. The results demonstrate the existence of an important developmentally regulated switch mediated by alternatively spliced exons in cardiac tissue at a time when major changes in excitation occur.

引用

页码：1497 / 1501

页数：5

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