BETA-1 INTEGRIN-MEDIATED COLLAGEN GEL CONTRACTION IS STIMULATED BY PDGF

被引:262
作者
GULLBERG, D
TINGSTROM, A
THURESSON, AC
OLSSON, L
TERRACIO, L
BORG, TK
RUBIN, K
机构
[1] BIOMED CTR,DEPT MED & PHYSIOL CHEM,BOX 575,S-75123 UPPSALA,SWEDEN
[2] UNIV UPPSALA,DEPT ZOOL,S-75122 UPPSALA,SWEDEN
[3] UNIV S CAROLINA,SCH MED,DEPT ANAT,COLUMBIA,SC 29208
[4] UNIV S CAROLINA,SCH MED,DEPT PATHOL,COLUMBIA,SC 29208
关键词
D O I
10.1016/0014-4827(90)90305-T
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The attachment of primary rat hepatocytes and fibroblasts to collagen type I is mediated by non-RGD-dependent β1 integrin matrix receptors. In this report we describe a novel 96-well microtiter plate assay for the quantification of fibroblast-mediated contraction of floating collagen type I gels. Fetal calf serum and platelet-derived growth factor (PDGF), but not transforming growth factor-β1, stimulated primary rat heart fibroblasts and normal human diploid fibroblasts (AG 1518) to contract collagen gels to less than 10% of the initial gel volume within a 24-h incubation period. Rabbit polyclonal antibodies directed to the rat hepatocyte integrin β1-chain inhibited the PDGF-stimulated collagen gel contraction. The inhibitory activity on contraction of the anti-β1 integrin IgG could be overcome by adding higher doses of PDGF. The contraction process was not blocked by anti-fibronectin IgG nor by synthetic peptides containing the tripeptide Arg-Gly-Asp (RGD), in concentrations that readily blocked fibroblast attachment to fibronectin-coated planar substrates. Autologous fibronectin or control peptides containing the tripeptide Arg-Gly-Glu were without effect. Immunofluorescence microscopy on fibroblasts grown within collagen gels revealed a punctate distribution of the β1 integrin and a lack of detectable levels of endogenously produced fibronectin. Collectively these data suggest a role for integrin collagen receptors with affinity for collagen fibers, distinct from the previously described RGD-dependent fibronectin receptors, in the fibronectin-independent PDGF-stimulated collagen gel contraction process. © 1990.
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页码:264 / 272
页数:9
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